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KMID : 0352519940310010135
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Korea Univercity Medical Journal
1994 Volume.31 No. 1 p.135 ~ p.148
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A Study of Renal Changes in experimentally Induced Diabetic Mice and the Effect of Early Insulin Thorapy on the Kidneys -Light microscopy, electron microscopy and immunofluorescence study-
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Abstract
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Diabetes mellitus is a common metabolic disease that injures multi-organs. In
particular, diabetic nephropathy, which is one of the main complications of death, progresses to end-stage renal disease for many years. The etiology of diabetic nephropathy, however, remains controversial whether it is due to
longstanding
hyperglvcemia per se, hemodynamic changes or others. Because most of the renal histopathologic studies had been done in long-term diabetic patients or animals,little investigation on the kidneys at early developing stage of diabetes
mellitus
is there. It is important to observe the earliest changes of diabetic nephropathy and the effect on the kidneys of insulin therapy and to investigate the correlation between hyperglycemia and renal histologic changes in its pathogenesis.
Therefore, an experimental study to observe the pathologic changes of the kidneys with light microscopy, electron microscopy, and immunofluorescence study, was performed on 15 control mice, 39 streptozotocin(STZ) induced diabetic mice(group
I),
20 insulin-treated diabetic mice(group II), which were sacrificed at 1,2,4,8, and 10 weeks in each group.
@ES The results are as follows.
1. There is no significant difference in the thickness of glomerular basement membranes between group I, diabetic mice and group II, insulin treated diabetic mice at 1,2,4,8 and 10 weeks after streptozotocin infection comparing to 15
normal
control mice at the same periods.
2. mesangial widening with increase of mesangial matrix was observed in group I diabetic mice (25/30) and in group II insulin treated diabetic mice (16/20) between 1 to 10 weeks of experiment.
3. Electron dense deposits are observed in mesangial regions in 13 of group I diabetic
mice(43%) and 9 of group II insulin treated diabetic mice(45%).
4. Immunofluorescence study shows positive reaction for IgG, IgM and IgA in mesangial
regions in a granular and diffuse pattern in 11 all of group I mice(100%) and 9 of group II mice(82%). And C3 is positive only in 4 of group I(37%) and 2 of group II mice(18%).
Taken the above results, mesangial widening with increase in mesangial matrix and electron dense deposits of immune complex fype are the earliest changes of diabetic nephropathy in streptozotocin induced diabetic mice. These changes are
diminished
by the early administration of insulin. Glomerular hypertrophy is followed thereafter, which is not changed by insulin therapy. Not as like as we knew, the thickness of glomerular basement membranes had not been changed until 10 weeks
after
streptozotocin injection in mice.
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